OfSPL11 Gene from Osmanthus fragrans Promotes Plant Growth and Oxidative Damage Reduction to Enhance Salt Tolerance in Arabidopsis

Osmanthus fragrans Lour. is a popular and traditional Chinese decorative plant. Salinity one of the major abiotic stresses affecting growth development O. fragrans. However, involvement SQUAMOSA PROMOTER BINDING PROTEIN-like (SPL) gene in salt stress response little understood. To elucidate role OfSPL genes resistance, we isolated candidate gene, OfSPL11, from genotype ‘Yanhong Gui’. OfSPL11 transcriptional activator that located nucleus. salt-inducible highly expressed young leaves shoots, according to tissue-specific expression external treatment. The promoter activity activated by treatments tobacco callus transgenic lines exhibited better physiological performance; under stress, have faster germination rate, longer roots, less leaf withering than wild type (WT). In addition, overexpression protected oxidative damage suppressing accumulation malondialdehyde (MDA) reactive oxygen species (ROSs) Arabidopsis. can promote some stresses, including AtCBL1, AtCOR15A, AtCOR6.6, AtRD29A, AtSOS2 AtSOS3. Yeast one-hybrid assays transient showed OfZAT12 (homologous Arabidopsis AtRHL41 gene) specifically binds positively regulates its expression. This study sheds fresh light on enhancing tolerance promoting reducing damage.